A04 Sjaak Philipsen/ Thamar van Dijk
Repurposing CRISPR/Cas9 for targeted demethylation of the gamma-globin promoters
Reactivation of fetal globin expression is clinically relevant for beta-hemoglobinopathy patients. We will determine whether DNA methylation of the fetal gamma-globin gene promoters is involved in their suppression in adult erythropoiesis. To achieve local DNA demethylation, we will take advantage of CRISPR/Cas9 technology to direct TET methylcytosine dioxygenases to the gamma-globin gene promoters. In addition, we will study the interplay of DNA methylation with putative fetal globin modulating pharmacological compounds, focussing on inhibitors of protein arginine methyltransferases.
| Name | Sjaak Philipsen |
|---|---|
| j.philipsen | |
| Telephone | +31 10 7044282 |
| Name | T.B. van Dijk |
|---|---|
| t.vandijk | |
| Telephone | +31 10 7044282 |