Publications

Börner J, Grützner J, Gerken F, Klug G. The Impact of the Major Endoribonucleases RNase E and RNase III and of the sRNA StsR on Photosynthesis Gene Expression in Rhodobacter sphaeroides Is Growth-Phase-Dependent. Int J Mol Sci. 2024 Aug 22;25(16):9123. doi: 10.3390/ijms25169123. PMID: 39201809; PMCID: PMC11354728.

Kretz J, Börner J, Friedrich T, McIntosh M, Procida-Kowalski T, Gerken F, Wilhelm J and Klug G. (2024) Function of the RNA-targeting class 2 type VI CRISPR Cas system of Rhodobacter capsulatus. Front. Microbiol. 2024 Apr. 29. 15:1384543. doi: 10.3389­/fmicb.­2024.­1384543

Börner J, Friedrich T, Klug G. RNase III participates in control of quorum sensing, pigmentation and oxidative stress resistance in Rhodobacter sphaeroides. Mol Microbiol. 2023 Oct 12. doi: 10.1111/mmi.15181. Epub ahead of print. PMID: 37823424.

Grützner J, Börner J, Jäger A, Klug G. The Small RNA-Binding Protein CcaF1 Promotes Formation of Photosynthetic Complexes in Rhodobacter sphaeroides. Int J Mol Sci. 2023 May 30;24(11):9515. doi: 10.3390/ijms24119515. PMID: 37298460; PMCID: PMC10253847.

Börner J, Friedrich T, Bartkuhn M, Klug G. Ribonuclease E strongly impacts bacterial adaptation to different growth conditions. RNA Biol. 2023 Jan;20(1):120-135. doi: 10.1080/15476286.2023.2195733. PMID: 36988476; PMCID: PMC10064930.

Spanka DT, Grützner J, Jäger A, Klug G. A Small RNA, UdsC, Interacts with the RpoHII mRNA and Affects the Motility and Stress Resistance of Rhodobacter sphaeroides. Int J Mol Sci. 2022 Dec 7;23(24):15486. doi: 10.3390/ijms232415486. PMID: 36555125; PMCID: PMC9779115.

Spanka DT, Klug G. Maturation of UTR-Derived sRNAs Is Modulated during Adaptation to Different Growth Conditions. Int J Mol Sci. 2021 Nov 12;22(22):12260. doi: 10.3390/ijms222212260. PMID: 34830143; PMCID: PMC8625941.

McIntosh M, Köchling T, Latz A, Kretz J, Heinen S, Konzer A, Klug G. A major checkpoint for protein expression in Rhodobacter sphaeroides during heat stress response occurs at the level of translation. Environ Microbiol. 2021 Nov;23(11):6483-6502. doi: 10.1111/1462-2920.15818. Epub 2021 Oct 19. PMID: 34668288

Eisenhardt KMH, Remes B, Grützner J, Spanka DT, Jäger A, Klug G. A Complex Network of Sigma Factors and sRNA StsR Regulates Stress Responses in R. sphaeroides. Int J Mol Sci. 2021 Jul 14;22(14):7557. doi: 10.3390/ijms22147557. PMID: 34299177; PMCID: PMC8307010.

Spanka DT, Klug G. Maturation of UTR-Derived sRNAs Is Modulated during Adaptation to Different Growth Conditions Int J Mol Sci. 2021 Nov 12;22(22):12260. doi: 10.3390/ijms222212260. PMID: 34830143 Free PMC article.

Reuscher CM, Klug G. Antisense RNA asPcrL regulates expression of photosynthesis genes in Rhodobacter sphaeroides by promoting RNase III-dependent turn-over of puf mRNA. RNA Biol. 2021 Oct;18(10):1445-1457. doi: 10.1080/15476286.2020.1857520. Epub 2021 Jan 11. PMID: 33258405

Eisenhardt KMH, Remes B, Grützner J, Spanka DT, Jäger A, Klug G. A Complex Network of Sigma Factors and sRNA StsR Regulates Stress Responses in R. sphaeroides. Int J Mol Sci. 2021 Jul 14;22(14):7557. doi: 10.3390/ijms22147557. PMID: 34299177; PMCID: PMC8307010.

Grützner J, Remes B, Eisenhardt KMH, Scheller D, Kretz J, Madhugiri R, McIntosh M, Klug G. sRNA-mediated RNA processing regulates bacterial cell division. Nucleic Acids Res. 2021 Jul 9;49(12):7035-7052. doi: 10.1093/nar/gkab491. PMID: 34125915; PMCID: PMC8266604

Wiegard JC, Schlüter MAC, Burenina OY, Kubareva EA, Klug G, Grünweller A, Hartmann RK. Northern Blot Detection of Tiny RNAs. Methods Mol Biol. 2021;2300:41-58. doi: 10.1007/978-1-0716-1386-3_5. PMID: 33792870

Grützner J, Billenkamp F, Spanka DT, Rick T, Monzon V, Förstner KU, Klug G. The small DUF1127 protein CcaF1 from Rhodobacter sphaeroides is an RNA-binding protein involved in sRNA maturation and RNA turnover. Nucleic Acids Res. 2021 Mar 12:gkab146. doi: 10.1093/nar/gkab146. Epub ahead of print. PMID: 33706375

Spanka DT, Reuscher CM, Klug G. Impact of PNPase on the transcriptome of Rhodobacter sphaeroides and its cooperation with RNase III and RNase E. BMC Genomics. 2021 Feb 6;22(1):106. doi: 10.1186/s12864-021-07409-4. PMID: 33549057; PMCID: PMC7866481

Reuscher CM, Klug G. Antisense RNA asPcrL regulates expression of photosynthesis genes in Rhodobacter sphaeroides by promoting RNase III-dependent turn-over of puf mRNA. RNA Biol. 2021 Jan 11:1-13. doi: 10.1080/15476286.2020.1857520. Epub ahead of print. PMID: 33258405

Bathke J, Gauernack AS, Rupp O, Weber L, Preusser C, Lechner M, Rossbach O, Goesmann A, Evguenieva-Hackenberg E, Klug G. iCLIP analysis of RNA substrates of the archaeal exosome. BMC Genomics. 2020 Nov 16;21(1):797. doi: 10.1186/s12864-020-07200-x. PMID: 33198623 Free PMC article.

Nie X, Jäger A, Börner J, Klug G. Interplay between formation of photosynthetic complexes and expression of genes for iron–sulfur cluster assembly in Rhodobacter sphaeroides? Photosynthesis Research, (), 1-10, 10.1007/s11120-020-00789-w

Licht MK, Nuss AM, Volk M, Konzer A, Beckstette M, Berghoff BA, Klug G. Adaptation to Photooxidative Stress: Common and Special Strategies of the Alphaproteobacteria Rhodobacter sphaeroides and Rhodobacter capsulatus. Microorganisms. 2020 Feb 19;8(2). pii: E283. doi: 10.3390/microorganisms8020283.

Nie X, Remes B, Klug G. Multiple Sense and Antisense Promoters Contribute to the Regulated Expression of the isc-suf Operon for Iron-Sulfur Cluster Assembly in Rhodobacter. Microorganisms. 2019 Dec 10;7(12). pii: E671. doi: 10.3390/microorganisms7120671.

Evguenieva-Hackenberg E, Gauernack AS, Hou L, Klug G. Enzymatic Analysis of Reconstituted Archaeal Exosomes. Methods Mol Biol. 2020;2062:63-79. doi: 10.1007/978-1-4939-9822-7_4.

Kubatova N, Pyper DJ, Jonker HRA, Saxena K, Remmel L, Richter C, Brantl S, Evguenieva-Hackenberg E, Hess WR, Klug G, Marchfelder A, Soppa J, Streit W, Mayzel M, Orekhov VY, Fuxreiter M, Schmitz RA, Schwalbe H. Rapid Biophysical Characterization and NMR Spectroscopy Structural Analysis of Small Proteins from Bacteria and Archaea. Chembiochem. 2019 Nov 9. doi: 10.1002/cbic.201900677.

McIntosh M, Eisenhardt K, Remes B, Konzer A, Klug G. Adaptation of the Alphaproteobacterium Rhodobacter sphaeroides to stationary phase Environ Microbiol. 2019 Nov;21(11):4425-4445. doi: 10.1111/1462-2920.14809. Epub 2019 Oct 17.

Bathke J, Konzer A, Remes B, McIntosh M, Klug G. Comparative analyses of the variation of the transcriptome and proteome of Rhodobacter sphaeroides throughout growth. BMC Genomics (2019) 20:358 https://doi.org/10.1186/s12864-019-5749-3

Zhao Z, Peng T, Oh JI, Glaeser J, Weber L, Li Q, Klug G. A response regulator of the OmpR family is part of the regulatory network controlling the oxidative stress response of Rhodobacter sphaeroides. Environ Microbiol Rep. 2018 Nov 19. doi: 10.1111/1758-2229.12718.]

Förstner KU, Reuscher CM, Haberzettl K, Weber L, Klug G. RNase E cleavage shapes the transcriptome of Rhodobacter sphaeroides and strongly impacts phototrophic growth. Life Science Alliance 2018 Aug. Vol. 1, No. 4. doi: 10.26508/lsa.201800080

Eisenhardt K, Reuscher C, Klug G. PcrX, an sRNA derived from the 3'- UTR of the Rhodobacter sphaeroides puf operon modulates expression of puf genes encoding proteins of the bacterial photosynthetic apparatus., Mol Microbiol. 2018 Jul 11. doi: 10.1111/mmi.14076.

Monakhova MV, Penkina AI, Pavlova AV, Lyaschuk AM, Kucherenko VV, Alexeevski AV, Lunin VG, Friedhoff P, Klug G, Oretskaya TS, Kubareva EA. Endonuclease Activity of MutL Protein of the Rhodobacter sphaeroides Mismatch Repair System. Biochemistry (Mosc). 2018 Mar;83(3):281-293. doi: 10.1134/S0006297918030082.

Li Q, Peng T, Klug G. The PhyR homolog RSP_1274 of Rhodobacter sphaeroides is involved in defense of membrane stress and has a moderate effect on RpoE (RSP_1092) activity. BMC Microbiol. 2018 Feb 27;18(1):18. doi: 10.1186/s12866-018-1161-4.

Gauernack AS, Lassek C, Hou L, Dzieciolowski J, Evguenieva-Hackenberg E, Klug G. Nop5 interacts with the archaeal RNA exosome. FEBS 2017 Dec;591(24):4039-4048. doi: 10.1002/1873-3468.12915. Epub 2017 Nov 28.

Ulyanova, V., Mahmud, R.S., Klug, G., Preissner, K.T., Vershinina, V., Ilinskaya, O. A set of genetic constructs for binase and barstar overproduction. BioNanoSci. 2017 doi:10.1007/s12668-016-0349-z

Elkina D, Weber L, Lechner M, Burenina O, Weisert A, Kubareva E, Hartmann RK, Klug G. 6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype. RNA Biol. Epub 2017 Jul 10:0. doi: 10.1080/15476286.2017.1342933.

Remes B, Rische-Grahl T, Müller KMH, Förstner KU, Yu SH, Weber L, Jäger A, Peuser V, Klug G. An RpoHI-Dependent Response Promotes Outgrowth after Extended Stationary Phase in the Alphaproteobacterium Rhodobacter sphaeroides. J Bacteriol. 2017 Jun 27;199(14). pii: e00249-17. doi: 10.1128/JB.00249-17. Print 2017 Jul 15.

Weber L, Thoelken C, Volk M, Remes B, Lechner M, Klug G. The Conserved Dcw Gene Cluster of R. sphaeroides Is Preceded by an Uncommonly Extended 5' Leader Featuring the sRNA UpsM. PLoS One. 2016 Nov 1;11(11)

von Zadow A, Ignatz E, Pokorny R, Essen LO, Klug G.  Rhodobacter sphaeroides CryB is a bacterial cryptochrome with (6-4) photolyase activity.G. FEBS J. 2016 Oct 14

Müller KM, Berghoff BA, Eisenhardt BD, Remes B, Klug G. Characteristics of Pos19 - A Small Coding RNA in the Oxidative Stress Response of Rhodobacter sphaeroides. PLoS One. 2016 Sep 26;11(9):e0163425. doi: 10.1371/journal.pone.0163425. eCollection 2016.

Peng T, Berghoff BA, Oh JI, Weber L, Schirmer J, Schwarz J, Glaeser J, Klug G. Regulation of a polyamine transporter by the conserved 3' UTR-derived sRNA SorX confers resistance to singlet oxygen and organic hydroperoxides in Rhodobacter sphaeroides.RNA Biol. 2016 Oct 2;13(10):988-999

Berghoff BA, Klug G (2016) An "omics" view on the response to singlet oxygen. In: Stress and Environmental Control of Gene Expression in Bacteria; de Bruijn FJ (Ed.), Volume 1, Wiley-Blackwell Publishers

Remes B, Eisenhardt BD, Srinivasan V, Klug G. IscR of Rhodobacter sphaeroides functions as repressor of genes for iron-sulfur metabolism and represents a new type of iron-sulfur-binding protein. Microbiologyopen. 2015 Aug 1

Adnan, F., Weber, L., Klug, G. (2015) The sRNA SorY confers resistance during photooxidative stress by affecting a metabolite transporter in Rhodobacter sphaeroides. RNA Biol. Apr 1:0. [Epub ahead of print]

Damm K, Bach S, Müller KM, Klug G, Burenina OY, Kubareva EA, Grünweller A, Hartmann RK. Improved Northern blot detection of small RNAs using EDC crosslinking and DNA/LNA probes. Methods Mol Biol. 2015;1296:41-51.

Damm K, Bach S, Müller KM, Klug G, Burenina OY, Kubareva EA, Grünweller A, Hartmann RK. Impact of RNA isolation protocols on RNA detection by Northern blotting. Methods Mol Biol. 2015;1296:29-38.

Billenkamp, F., Peng, T., Berghoff, B.A., Klug, G. (2015) A cluster of four homologous small RNAs modulates C1 metabolism and the pyruvate dehydrogenase complex in Rhodobacter sphaeroides under various stress conditions. J. Bacteriol. 197: 1839-1852

Damm, K., Bach, S., Müller, K.M., Klug, G. Burenina, O.Y., Kubareva, E.A. Grünweller, A., Hartmann, R. (2015) Improved northern blot detection of small RNAs using EDC crosslinking and DNA/LNA probes. Methods Mol. Biol. 1296:41-51

Damm, K., Bach, S., Müller, K.M., Klug, G., Burenina, O.Y., Kubareva, E.A. Grünweller, A., Hartmann, R. (2015) Impact of RNA isolation protocols on RNA detection by northern blotting. Methods Mol. Biol. 1296:29-38

Remes B, Berghoff BA, Förstner KU, Klug G (2014) Role of oxygen and the OxyR protein in the response to iron limitation in Rhodobacter sphaeroides. BMC Genomics 15: 794

Rische-Grahl T, Weber L, Remes B, Förstner KU, Klug G. (2014) RNase J is required for processing of a small number of RNAs in Rhodobacter sphaeroides. RNA Biol. 12;11(7)

Evguenieva-Hackenberg E, Hou L, Glaeser S, Klug G. (2014) Structure and function of the archaeal exosome. Wiley Interdiscip Rev RNA. 2014 Apr 30. doi: 10.1002/wrna.1234.

Hess, W., Berghoff, B., Wilde, A., Steglich, C., Klug, G. (2014) Riboregulators and the role of Hfq in photosynthetic bacteria. RNA Biology 11: 413-426

Klug G. (2014) Beyond catalysis: vitamin B12 as a cofactor in gene regulation. Mol Microbiol. Feb;91(4):635-40.

Hou, L., Klug, G., Evguenieva-Hackenberg, E. (2013) The archaeal DnaG protein needs Csl4 for binding to the exosome and enhances its interaction with adenine-rich RNA. RNA Biology 10: 415-424

Zarkani, A.A., Stein, E., Röhrich, C.R., Schikora, M., Evguenieva-Hackenberg, E., Degenkolb, T., Vilcinskas, A., Klug, G., Kogel, K.-H., Schikora, A. (2013) Homoserine lactones govern the reaction of plants to rhizobia. Int. J. Mol. Sci. 14: 17122-17146

Nuss, A.M., Adnan, F., Weber, L., Berghoff, B.A., Glaeser, J., Klug, G. (2013) DegS and RseP homologous proteases are involved in singlet oxygen dependent activation of RpoE in Rhodobacter sphaeroides. PLoS One. 2013 Nov 5;8(11):e79520. doi: 10.1371/journal.pone.0079520. eCollection 2013.

Berghoff, B.A., Konzer, A., Mank, N.N., Looso, M., Rische, T., Förstner, K.U., Krüger, M., Klug, G. (2013) Integratives "Omics"-approach discovers dynamic and regulatory features of bacterial stress responses. PLoS Genetics 10.1371/journal.pgen.1003576

Mank, N. N., Berghoff, B. A., Klug, G. (2013) A mixed incoherent feed-forward loop contributes to the regulation of bacterial photosynthesis genes. RNA Biol. 2013 Mar;10(3):347-52

Mank,N. N., Berghoff, B. A., Hermanns, Y. N., Klug, G. (2012) Regulation of bacterial photosynthesis genes by small non-coding RNA PcrZ. Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16306-11. doi: 10.1073/pnas.1207067109. Epub 2012 Sep 17.

Peuser, V., Remes, B., Klug, G. (2012) Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides. PLoS ONE 7: e42231, doi:10.1371/journal.pone.0042231

Witharana, C., Roppelt, V., Lochnit, G., Klug, G., Evguenieva-Hackenberg E. (2012) Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus. Biochimie 94: 1578-1587.

Frühwirth, S., Teich, K., Klug, G. (2012) Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression the dark or blue light or in presence of singlet oxygen. PLoS ONE doi:10.1371/journal.pone.0033791

Metz, S., Haberzettl, K., Fruehwirth, S.W., Teich, K., Hasewinkel, C., Klug, G. (2012) Interaction of two photoreceptors, AppA and CryB, in the regulation of photosynthesis genes in Rhodobacter sphaeroides. Nucleic Acids Res. doi: 10.1093/nar/gks243

Rische, T., Klug, G. (2012) The ordered processing of intervening sequences in 23S rRNA of Rhodobacter sphaeroides requires RNase J. RNA Biology 9: 343-350

Madhugiri R, Pessi G, Voss B, Hahn J, Sharma CM, Reinhardt R, Vogel J, Hess WR, Fischer HM, Evguenieva-Hackenberg E. (2012) Small RNAs of the Bradyrhizobium/Rhodopseudomonas lineage and their analysis. RNA Biol. 2012 Jan 1;9(1). [Epub ahead of print]

Geisselbrecht, Y., Frühwirth, S., Schröder, C., Pierik, A. J., Klug, G., Essen, L.-O. (2012) CryB from Rhodobacter sphaeroides: A unique class of cryptochromes with novel cofactors. EMBO reports13: 223-229

Metz, S., Jäger, A., Klug, G. (2012) Role of a short LOV protein in blue light- and singlet oxygen-dependent gene regulation in Rhodobacter sphaeroides. Microbiology 158: 353-352

Glaeser, J., Nuss, A.M., Berghoff, B.A., Klug, G. (2011) Singlet oxygen stress in microorganisms. In R. Poole, R.K. (ed): Adv. in Microb. Physiol. Vol. 58 (Amsterdam), pp. 141-173

Evguenieva-Hackenberg, E., Klug, G. (2011) New aspects of RNA processing in prokaryotes. Curr. Opin. Microbiol. 14: 587-592

Peuser, V. Glaeser, J. Klug, G. (2011) The RSP2889 gene product of Rhodobacter sphaeroides is a CueR homolog controlling copper responsive genes. Microbiology 157: 3306-3323

Peuser, V., Metz, S., Klug, G. (2011) Response of the facultatively photosynthetic bacterium Rhodobacter sphaeroides to iron limitation and the role of a Fur ortholog in this response. Environment. Microbiol. Rep. 3: 397-404

Berghoff, B. A., Klug, G. (2011) Small RNAs with a role in the oxidative stress response in bacteria. In: Marchfelder, A., Hess, W. eds.: Regulatory RNAs in prokaryotes. Springer Verlag.

Berghoff, B.A., Glaeser, J., Sharma, C.M., Zobawa, M., Lottspeich, F., Vogel, J., Klug, G. (2011) Contribution of Hfq to photooxidative stress resistance and global regulation in Rhodobacter sphaeroides. Mol. Microbiol. 80: 1479-1495

Berghoff, B.A., Glaeser, J., Nuss, A.N., Zobawa, M., Lottspeich, F., Klug, G. (2011) Anoxygenic photosynthesis and photooxidative stress: A particular challenge for Roseobacter. Environm. Microbiol.

Evguenieva-Hackenberg, E., Roppelt, V., Lassek, C., Klug, G. (2011) Subcellular localisation of RNA degrading proteins and protein complexes in prokaryotes. RNA Biology 8: 49-54

Madhugiri, R., Basineni, S.R., Klug, G. Turn-over of the small non-coding RNA RprA in E. coli is influenced by osmolarity. Mol Genet Genomics. 2010 284:307-18 Mol Genet Genomics. 2010 Oct;284(4):307-18. Epub 2010 Aug 18.

Roppelt, V., Klug, G., Evguenieva-Hackenberg, E. The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome. FEBS Lett. 2010 584: 2931-2936

Roppelt, V., Hobel, C.F., Albers, S.V., Lassek, C., Schwarz, H., Klug, G., Evguenieva-Hackenberg, E. The archaeal exosome localizes to the membrane. FEBS Lett. 2010 584:2791-5

Metz, S., Hendriks, J., Jäger, A. Hellingwerf, J., Klug, G. In vivo effects on photosynthesis gene expression of base pair exchanges in the gene encoding the light responsive BLUF domain of AppA in Rhodobacter sphaeroides. Photochem. Photobiol. 2010 86:882-9

Nuss, A.M., Glaeser, J., Berghoff, B.A., Klug, G. Overlapping alternative sigma factor regulons in the response to singlet oxygen in Rhodobacter sphaeroides. J Bacteriol 2010 192:2613-23

Weisel, J., Wagner, S., Klug, G. The Nop5-L7A-fibrillarin RNP complex and a novel box C/D containing sRNA of Halobacterium salinarum NRC-1. 2010 394:542-7

Berghoff, B.A., Glaeser, J., Sharma, C., Jörg Vogel, J., Klug, G. Photooxidative stress induced and abundant small RNAs in Rhodobacter sphaeroides. Mol Microbiol. 2009 74:1497-512.

Hendrischk, A-K.,Frühwirth, S.W., Moldt, J, Pokorny, R., Metz, S., Kaiser, G., Jäger, A, Batschauer, A., Klug, G. A cryptochrome-like protein is involved in the regulation of photosynthesis genes in Rhodobacter sphaeroides. Mol Microbiol 2009 74:990-1003

Basineni, S. R., Madhugiri, R., Kolmsee, T., Hengge, R., Klug, G. The influence of Hfq and ribonucleases on the stability of the small non-coding RNA OxyS and its target rpoS in E. coli is growth phase dependent. RNA Biol. 2009 6:584-94.

Hendrischk, A-K., Moldt, J., Frühwirth, S.W., Klug, G. Characterization of an unusual LOV domain protein in the alpha-proteobacterium Rhodobacter sphaeroides. Photochem. Photobiol. 2009 85: 1254-1259

Metz, S., Jäger, A., Klug, G. In vivo studies on the light sensitivity of the photosynthetic gene regulators AppA/PpsR and PrrA/PrrB in Rhodobacter sphaeroides. J. Bacteriol. 2009 191: 4473-4477

Evguenieva-Hackenberg, E., Klug, G. RNA degradation in Archaea and Gram-negative bacteria different from Escherichia coli. In: Molecular Biology of RNA Processing and Decay in Prokaryotes, Condon, C. ed. Prog. Mol. Biol. and Transl. Sci. 2009 85: 275-317

Nuss AM, Glaeser J, Klug G. RpoH(II) activates oxidative-stress defense systems and is controlled by RpoE in the singlet oxygen-dependent response in Rhodobacter sphaeroides. J Bacteriol. 2009 191(1):220-30

Evguenieva-Hackenberg E, Wagner S, Klug G. In vivo and in vivo studies on RNA degrading activities in Archea. In: RNA turn-over in Bacteria, Archea and Organelles. Maquat LE, Arraiano CM. eds Methods in Enzymol. 2008 447:381-416

Evguenieva-Hackenberg E, Roppelt V, Finsterseifer P, Klug G. Rrp4 and Csl4 are needed for efficient degradation but not for polyadenylation of synthetic and natural RNA by the archaeal exosome. Biochemistry. 2008 16;47(50):13158-68.

Klug G, Masuda S. Regulation of genes by light. In: The purple phototrophic Bacteria. Advances in Photosynthesis and Respiration. eds Hunter CN, Daldal F, Thurnauer M, Beatty JT. Springer, Dodrecht, Netherlands. 2008 Vol 28 pp.155-179

Zeller T, Klug G. Bacterial thioredoxins: genes and regulation. In: Genes, Genomes and Genomics. Global Sc. Books, UK. 2008 1(2):226-231 2008

Hundt S, Zaigler A, Lange C, Soppa J, Klug G. Global analysis of mRNA decay in Halobacterium salinarum NRC-1 at single-gene resolution using DNA microarrays. J Bacteriol. 2007 Oct; 189(19):6936-44

Gregor J, Zeller T, Balzer A, Haberzettl K, Klug G. Bacterial regulatory networks include direct contact of response regulator proteins: interaction of RegA and NtrX in Rhodobacter capsulatus. J Mol Microbiol Biotechnol. 2007;13(1-3):126-39

Glaeser J, Zobawa M, Lottspeich F, Klug G. Protein synthesis patterns reveal a complex regulatory response to singlet oxygen in Rhodobacter. J Proteome Res. 2007 Jul; 6:2460-71

Wagner S, Klug G. An archaeal protein with homology to the eukaryotic translation initiation factor 5A shows ribonucleolytic activity. J Biol Chem. 2007 May; 282(19):13966-76

Adhikary, S., Klug, G. Detection of a gene for iron containing superoxide dismutase in the terrestrial cyanobacterium Tolypothrix byssoidea. Ind. J. Biotechn. 2007 6: 118-120

Hendrischk AK, Braatsch S, Glaeser J, Klug G. The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a {sigma}E-dependent manner. Microbiology. 2007 Jun;153(6):1842-51.

Zeller T, Mraheil MA, Moskvin OV, Li K, Gomelsky M, Klug G. Regulation of Hydrogen Peroxide-Dependent Gene Expression in Rhodobacter sphaeroides: Regulatory Functions of OxyR. J Bacteriol. 2007 May;189(10):3784-92.

Han Y, Meyer MH, Keusgen M, Klug G. A haem cofactor is required for redox and light signalling by the AppA protein of Rhodobacter sphaeroides. Mol Microbiol. 2007 May;64(4):1090-104.

Jager A, Braatsch S, Haberzettl K, Metz S, Osterloh L, Han Y, Klug G. The AppA and PpsR proteins from Rhodobacter sphaeroides can establish a redox dependent signal chain but fail to transmit blue light signals in other bacteria. J Bacteriol. 2007 Mar;189(6):2274-82

Walter P, Klein F, Lorentzen E, Ilchmann A, Klug G, Evguenieva-Hackenberg E. Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus. Mol Microbiol. 2006 Nov;62(4):1076-89.

Zeller T, Li K, Klug G. Expression of the trxC gene of Rhodobacter capsulatus: response to cellular redox status is mediated by the transcriptional regulator OxyR. J Bacteriol. 2006 Nov;188(21):7689-95.

Zeller T, Klug G. Thioredoxins in bacteria: functions in oxidative stress response and regulation of thioredoxin genes. Naturwissenschaften. 2006 Jun;93(6):259-66. Review

Portnoy V, Evguenieva-Hackenberg E, Klein F, Walter P, Lorentzen E, Klug G, Schuster G. RNA polyadenylation in Archaea: not observed in Haloferax while the exosome polynucleotidylates RNA in Sulfolobus. EMBO Rep. 2005 Dec;6(12):1188-93.

Happ HN, Braatsch S, Broschek V, Osterloh L, Klug G. Light-dependent regulation of photosynthesis genes in Rhodobacter sphaeroides 2.4.1 is coordinately controlled by photosynthetic electron transport via the PrrBA two-component system and the photoreceptor AppA. Mol Microbiol. 2005 Nov;58(3):903-14.

Zeller T, Moskvin OV, Li K, Klug G, Gomelsky M. Transcriptome and physiological responses to hydrogen peroxide of the facultatively phototrophic bacterium Rhodobacter sphaeroides. J Bacteriol. 2005 Nov;187(21):7232-42.

Braatsch S, Klug G. Blue light perception in bacteria. Photosynth Res. 2004;79(1):45-57.

Braatsch S, Klug G. ORF90, a Gene Required for Photoreactivation in Rhodobacter capsulatus SB1003 Encodes a Cyclobutane Pyrimidine Dimer Photolyase. Photosynth Res. 2004;79(2):167-77.

Lorentzen E, Walter P, Fribourg S, Evguenieva-Hackenberg E, Klug G, Conti E. The archaeal exosome core is a hexameric ring structure with three catalytic subunits. Nat Struct Mol Biol. 2005 Jul;12(7):575-81.

Glaeser J, Klug G. Photo-oxidative stress in Rhodobacter sphaeroides: protective role of carotenoids and expression of selected genes. Microbiology. 2005 Jun;151(Pt 6):1927-38.

Purusharth RI, Klein F, Sulthana S, Jager S, Jagannadham MV, Evguenieva-Hackenberg E, Ray MK, Klug G. Exoribonuclease R interacts with endoribonuclease E and an RNA helicase in the psychrotrophic bacterium Pseudomonas syringae Lz4W. J Biol Chem. 2005 Apr 15;280(15):14572-8.

Braatsch S, Moskvin OV, Klug G, Gomelsky M. Responses of the Rhodobacter sphaeroides transcriptome to blue light under semiaerobic conditions. J Bacteriol. 2004 Nov;186(22):7726-35.

Zeller T, Klug G. Detoxification of hydrogen peroxide and expression of catalase genes in Rhodobacter. Microbiology. 2004 Oct;150(Pt 10):3451-62.

Li K, Hein S, Zou W, Klug G. The glutathione-glutaredoxin system in Rhodobacter capsulatus: part of a complex regulatory network controlling defense against oxidative stress. J Bacteriol. 2004 Oct;186(20):6800-8.

Li K, Pasternak C, Hartig E, Haberzettl K, Maxwell A, Klug G. Thioredoxin can influence gene expression by affecting gyrase activity. Nucleic Acids Res. 2004 Aug 24;32(15):4563-75.

Han Y, Braatsch S, Osterloh L, Klug G. A eukaryotic BLUF domain mediates light-dependent gene expression in the purple bacterium Rhodobacter sphaeroides 2.4.1. Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12306-11.

Jager S, Hebermehl M, Schiltz E, Klug G. Composition and activity of the Rhodobacter capsulatus degradosome vary under different oxygen concentrations. J Mol Microbiol Biotechnol. 2004;7(3):148-54.

Jager S, Evguenieva-Hackenberg E, Klug G. Temperature-dependent processing of the cspA mRNA in Rhodobacter capsulatus. Microbiology. 2004 Mar;150(Pt 3):687-95.

Jager S, Jager A, Klug G. CIRCE is not involved in heat-dependent transcription of groESL but in stabilization of the mRNA 5'-end in Rhodobacter capsulatus. Nucleic Acids Res. 2004 Jan 16;32(1):386-96.

Li K, Pasternak C, Klug G. Expression of the trxA gene for thioredoxin 1 in Rhodobacter sphaeroides during oxidative stress. Arch Microbiol. 2003 Dec;180(6):484-9.

Evguenieva-Hackenberg E, Walter P, Hochleitner E, Lottspeich F, Klug G. An exosome-like complex in Sulfolobus solfataricus. EMBO Rep. 2003 Sep;4(9):889-93.

Li K, Hartig E, Klug G. Thioredoxin 2 is involved in oxidative stress defence and redox-dependent expression of photosynthesis genes in Rhodobacter capsulatus. Microbiology. 2003 Feb;149(Pt 2):419-30.

Jager A, Samorski R, Pfeifer F, Klug G. Individual gvp transcript segments in Haloferax mediterranei exhibit varying half-lives, which are differentially affected by salt concentration and growth phase. Nucleic Acids Res. 2002 Dec 15;30(24):5436-43.

Gomelsky M, Klug G. BLUF: a novel FAD-binding domain involved in sensory transduction in microorganisms. Trends Biochem Sci. 2002 Oct;27(10):497-500. Review.

Evguenieva-Hackenberg E, Schiltz E, Klug G. Dehydrogenases from all three domains of life cleave RNA. J Biol Chem. 2002 Nov 29;277(48):46145-50.

Braatsch S, Gomelsky M, Kuphal S, Klug G. A single flavoprotein, AppA, integrates both redox and light signals in Rhodobacter sphaeroides. Mol Microbiol. 2002 Aug;45(3):827-36.

Klein F, Samorski R, Klug G, Evguenieva-Hackenberg E. Atypical processing in domain III of 23S rRNA of Rhizobium leguminosarum ATCC 10004(T) at a position homologous to an rRNA fragmentation site in protozoa. J Bacteriol. 2002 Jun;184(12):3176-85.

Conrad C, Schmitt JG, Evguenieva-Hackenberg E, Klug G. One functional subunit is sufficient for catalytic activity and substrate specificity of Escherichia coli endoribonuclease III artificial heterodimers. FEBS Lett. 2002 May 8;518(1-3):93-6.

Abada EM, Balzer A, Jager A, Klug G. Bacteriochlorophyll-dependent expression of genes for pigment-binding proteins in Rhodobacter capsulatus involves the RegB/RegA two-component system. Mol Genet Genomics. 2002 Apr;267(2):202-9.

Gregor J, Klug G. Oxygen-regulated expression of genes for pigment binding proteins in Rhodobacter capsulatus. J Mol Microbiol Biotechnol. 2002 May;4(3):249-53. Review.

Conrad C, Evguenieva-Hackenberg E, Klug G. Both N-terminal catalytic and C-terminal RNA binding domain contribute to substrate specificity and cleavage site selection of RNase III. FEBS Lett. 2001 Nov 30;509(1):53-8.

Jager S, Fuhrmann O, Heck C, Hebermehl M, Schiltz E, Rauhut R, Klug G. An mRNA degrading complex in Rhodobacter capsulatus. Nucleic Acids Res. 2001 Nov 15;29(22):4581-8.

Evguenieva-Hackenberg E, Klug G. RNase III processing of intervening sequences found in helix 9 of 23S rRNA in the alpha subclass of Proteobacteria. J Bacteriol. 2000 Sep;182(17):4719-29.

Hemschemeier SK, Kirndorfer M, Hebermehl M, Klug G. DNA binding of wild type RegA protein and its differential effect on the expression of pigment binding proteins in Rhodobacter capsulatus. J Mol Microbiol Biotechnol. 2000 Apr;2(2):235-43.

Hemschemeier SK, Ebel U, Jager A, Balzer A, Kirndorfer M, Klug G. In vivo and in vitro analysis of RegA response regulator mutants of Rhodobacter capsulatus. J Mol Microbiol Biotechnol. 2000 Jul;2(3):291-300.

Chen W, Jager A, Klug G. Correction of the DNA sequence of the regB gene of Rhodobacter capsulatus with implications for the membrane topology of the sensor kinase RegB. J Bacteriol. 2000 Feb;182(3):818-20.

Heck C, Balzer A, Fuhrmann O, Klug G. Initial events in the degradation of the polycistronic puf mRNA in Rhodobacter capsulatus and consequences for further processing steps. Mol Microbiol. 2000 Jan;35(1):90-100.

Heck C, Evguenieva-Hackenberg E, Balzer A, Klug G. RNase E enzymes from Rhodobacter capsulatus and Escherichia coli differ in context- and sequence-dependent in vivo cleavage within the polycistronic puf mRNA. J Bacteriol. 1999 Dec;181(24):7621-5.

Hemschemeier, S.K., Kirndörfer, M., Ebel, U., Klug, G. (1999) Transcriptional regulation of puf and puc expression in Rhodobacter capsulatus by the DNA binding protein RegA. In: Peschek, G.A., Löffelhardt, W., Schmetterer, G., The Phototrophic Prokaryotes. Plenum Press 17-130

Gregor J, Klug G. Regulation of bacterial photosynthesis genes by oxygen and light. FEMS Microbiol Lett. 1999 Oct 1;179(1):1-9. Review.

Rauhut R, Klug G. mRNA degradation in bacteria. FEMS Microbiol Rev. 1999 Jun;23(3):353-70. Review.

Pasternak C, Haberzettl K, Klug G. Thioredoxin is involved in oxygen-regulated formation of the photosynthetic apparatus of Rhodobacter sphaeroides. J Bacteriol. 1999 Jan;181(1):100-6.

Emetz D, Klug G. Cloning and characterization of the rpoH gene of Rhodobacter capsulatus. Mol Gen Genet. 1998 Nov;260(2-3):212-7.

Cortez N, Carrillo N, Pasternak C, Balzer A, Klug G. Molecular cloning and expression analysis of the Rhodobacter capsulatus sodB gene, encoding an iron superoxide dismutase. J Bacteriol. 1998 Oct;180(20):5413-20.

Conrad C, Rauhut R, Klug G. Different cleavage specificities of RNases III from Rhodobacter capsulatus and Escherichia coli. Nucleic Acids Res. 1998 Oct 1;26(19):4446-53.

Hebermehl M, Klug G. Effect of oxygen on translation and posttranslational steps in expression of photosynthesis genes in Rhodobacter capsulatus. J Bacteriol. 1998 Aug;180(15):3983-7.

Kirndorfer M, Jager A, Klug G. Integration host factor affects the oxygen-regulated expression of photosynthesis genes in Rhodobacter capsulatus. Mol Gen Genet. 1998 May;258(3):297-305.

Klug G, Jager A, Heck C, Rauhut R. Identification, sequence analysis, and expression of the lepB gene for a leader peptidase in Rhodobacter capsulatus. Mol Gen Genet. 1997 Feb 27;253(6):666-73.

Pasternak C, Assemat K, Clement-Metral JD, Klug G. Thioredoxin is essential for Rhodobacter sphaeroides growth by aerobic and anaerobic respiration. Microbiology. 1997 Jan;143 (Pt 1):83-91.

Pollich M, Wersig C, Klug G. The bluF gene of Rhodobacter capsulatus is involved in conversion of cobinamide to cobalamin (vitamin B12). J Bacteriol. 1996 Dec;178(24):7308-10.

Pasternak C, Chen W, Heck C, Klug G. Cloning, nucleotide sequence and characterization of the rpoD gene encoding the primary sigma factor of Rhodobacter capsulatus. Gene. 1996 Oct 17;176(1-2):177-84.

Heck C, Rothfuchs R, Jager A, Rauhut R, Klug G. Effect of the pufQ-pufB intercistronic region on puf mRNA stability in Rhodobacter capsulatus. Mol Microbiol. 1996 Jun;20(6):1165-78.

Rauhut R, Jager A, Conrad C, Klug G. Identification and analysis of the rnc gene for RNase III in Rhodobacter capsulatus. Nucleic Acids Res. 1996 Apr 1;24(7):1246-51.

Pasternak C, Assemat K, Breton AM, Clement-Metral JD, Klug G. Expression of the thioredoxin gene (trxA) in Rhodobacter sphaeroides Y is regulated by oxygen. Mol Gen Genet. 1996 Feb 5;250(2):189-96.

Pollich M, Klug G. Identification and sequence analysis of genes involved in late steps in cobalamin (vitamin B12) synthesis in Rhodobacter capsulatus. J Bacteriol. 1995 Aug;177(15):4481-7.

Fritsch J, Rothfuchs R, Rauhut R, Klug G. Identification of an mRNA element promoting rate-limiting cleavage of the polycistronic puf mRNA in Rhodobacter capsulatus by an enzyme similar to RNase E. Mol Microbiol. 1995 Mar;15(6):1017-29.

Kordes E, Jock S, Fritsch J, Bosch F, Klug G. Cloning of a gene involved in rRNA precursor processing and 23S rRNA cleavage in Rhodobacter capsulatus. J Bacteriol. 1994 Feb;176(4):1121-7.

Pollich M, Jock S, Klug G. Identification of a gene required for the oxygen-regulated formation of the photosynthetic apparatus of Rhodobacter capsulatus. Mol Microbiol. 1993 Nov;10(4):749-57.

Klug G. The role of mRNA degradation in the regulated expression of bacterial photosynthesis genes. Mol Microbiol. 1993 Jul;9(1):1-7. Review.

Klug G. Regulation of expression of photosynthesis genes in anoxygenic photosynthetic bacteria. Arch Microbiol. 1993;159(5):397-404. Review. Erratum in: Arch Microbiol 1993;160(4):332.

Klug G, Jock S, Rothfuchs R. The rate of decay of Rhodobacter capsulatus-specific puf mRNA segments is differentially affected by RNase E activity in Escherichia coli. Gene. 1992 Nov 2;121(1):95-102.

Klug G, Jock S. A base pair transition in a DNA sequence with dyad symmetry upstream of the puf promoter affects transcription of the puc operon in Rhodobacter capsulatus. J Bacteriol. 1991 Oct;173(19):6038-45.

Klug G, Gad'on N, Jock S, Narro ML. Light and oxygen effects share a common regulatory DNA sequence in Rhodobacter capsulatus. Mol Microbiol. 1991 May;5(5):1235-9.

Klug G. A DNA sequence upstream of the puf operon of Rhodobacter capsulatus is involved in its oxygen-dependent regulation and functions as a protein binding site. Mol Gen Genet. 1991 Apr;226(1-2):167-76.

Klug G. Endonucleolytic degradation of puf mRNA in Rhodobacter capsulatus is influenced by oxygen. Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1765-9.

Klug G, Cohen SN. Effects of translation on degradation of mRNA segments transcribed from the polycistronic puf operon of Rhodobacter capsulatus. J Bacteriol. 1991 Feb;173(4):1478-84.

Stiehle H, Cortez N, Klug G, Drews G. A negatively charged N terminus in the alpha polypeptide inhibits formation of light-harvesting complex I in Rhodobacter capsulatus. J Bacteriol. 1990 Dec;172(12):7131-7.

Klug G, Cohen SN. Combined actions of multiple hairpin loop structures and sites of rate-limiting endonucleolytic cleavage determine differential degradation rates of individual segments within polycistronic puf operon mRNA. J Bacteriol. 1990 Sep;172(9):5140-6.

Dorge B, Klug G, Gad'on N, Cohen SN, Drews G. Effects on the formation of antenna complex B870 of Rhodobacter capsulatus by exchange of charged amino acids in the N-terminal domain of the alpha and beta pigment-binding proteins. Biochemistry. 1990 Aug 21;29(33):7754-8.

Klug G, Cohen SN. Pleiotropic effects of localized Rhodobacter capsulatus puf operon deletions on production of light-absorbing pigment-protein complexes. J Bacteriol. 1988 Dec;170(12):5814-21.

Klug G, Adams CW, Belasco J, Doerge B, Cohen SN. Biological consequences of segmental alterations in mRNA stability: effects of deletion of the intercistronic hairpin loop region of the Rhodobacter capsulatus puf operon. EMBO J. 1987 Nov;6(11):3515-20.

Klug G, Kaufmann N, Drews G. Gene expression of pigment-binding proteins of the bacterial photosynthetic apparatus: Transcription and assembly in the membrane of Rhodopseudomonas capsulata. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6485-6489.

Klug G, Drews G. Construction of a gene bank of Rhodopseudomonas capsulata using a broad host range DNA cloning system. Arch Microbiol. 1984 Nov;139(4):319-25.