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Dr. Martin Gand

   

Dr. Martin Gand


Junior Research Group Leader 

 

Phone: +49 641 / 99 34912

Fax: +49 641 / 99 34909

Office: B 239

 

 


As part of my current work, I am mainly investigating the use of enzymes in food production. Examples of this include the use of lipases in cheese production, for the generation of piquant flavors or the use of peptidases for the degradation of haze-forming proteins in wine. The use of fungi of the Basidiomycota for the production of novel colorants is also part of my work. In addition, I am working on the utilization of mycelium as food or animal feed.

 

Work Priorities

  • Homologous and heterologous expression of enzymes, as well as the identification of new enzymes from fungi and insects
  • Purification and stabilization of enzyme preparations
  • Use of side streams and renewable raw materials for the cost-effective production of enzymes
  • Production of aroma-active substances using (chemical) enzymatic processes
  • Use of -omics data for the identification of enzymes
  • Structural elucidation of colorants and transformation products
  • Production of edible mushroom mycelium on the basis of food side stream

 

Curriculum Vitae

  • since 2018 Postdoc at the Justus Liebig University Giessen in the working group for food chemistry and food biotechnology of Prof. Dr. Holger Zorn
  • 2016-2018 Postdoc at the University of Hamburg in the working group for molecular phytopathology of Prof. Dr. Wilhelm Schäfer, BMBF project: New Enzymes and Metabolites
  • 2012-2016 Doctorate at the University of Greifswald in the working group for protein biochemistry of Jun. Prof. Dr. Matthias Höhne; Topic: "Identification and characterization of imine reductases"
  • 2007-2012 Study of biochemistry at the University of Greifswals, thesis in the working group for biotechnology and enzyme catalysis of Prof. Dr. Uwe Bornscheuer

 

Reviewer for international journals

  • Journal of Agricultural and Food Chemistry / Foods / Enzyme and Microbial Technology / Proteins and Proteomics / Applied Sciences / Bioscience Reports / Biotechnology Progress / Experimental Results / International Journal of Molecular Sciences / Frontiers in Catalysis

 

Societies

  • Since 01/2024    Member of German Society for Fat Sciences (DGF)
  • Since 09/2022    Member of the future forum at the DECHEMA
  • Since 03/2022    Member of the Professional Association for Education and Innovation, Natural Sciences Division of the DECHEMA
  • Since 11/2020    Member of Advisory Board of the Expert Group of Food Biotechnologists of the DECHEMA
  • Since 05/2020    Member of DECHEMA, Society for Chemical Engineering and Biotechnology e.V.

 

Publications: Peer reviewed journals

[25]

Novel catechol O-methyltransferases from Lentinula edodes catalyse the generation of taste-active flavonoids

Kanter JP, Milke L, Metz JK, Biabani A, Schlüter H, Gand M, Ley JP, Zorn H, J Agric Food Chem, 2024, DOI: 10.1021/acs.jafc.4c01514

[24]

MIF-like domain containing protein orchestrates cellular differentiation and virulence in the fungal pathogen Magnaporthe oryzae

Galli M, Jacob S, Zheng Y, Ghezellou P, Gand M, Albuquerque W, Imani J, Allasia V, Coustau C, Keller H, Spengler B, Thines E, Kogel KH, iScience, 2023, DOI:10.1016/j.isci.2023.107565

[23]

Mass spectrometry-based proteomic profiling of a Silvaner wine

Albuquerque W, Ghezellou P, Seidel L, Burkert L, Will F, Schweiggert R, Spengler B, Zorn H, Gand M, Biomolecules, 2023, DOI:10.3390/biom13040650

[22]

Peptidomics as a tool to assess the cleavage of wine haze proteins by peptidases from Drosophila suzukii larvae

Albuquerque W, Ghezellou P, Lee KZ, Schneider Q, Gross P, Kessel T, Omokungbe B, Spengler B, Vilcinskas A, Zorn H, Gand M, Biomolecules, 2023, DOI:10.3390/biom13030451

[21] 

Composition, zeta potential and molar mass distribution of 20 must and wine colloids from five different varieties obtained during four consecutive years.

Seidel L, Albuquerque W, Happel K, Ghezellou P, Gand M, Spengler B, Zorn H, Will F, Schweiggert R, J Agric Food Chem, 2023, DOI:10.1021/acs.jafc.2c09048

[20]

Recombinant thaumatin-like protein (rTLP) and chitinase (rCHI) from Vitis vinifera as models for wine haze formation.

Albuquerque W, Sturm C, Schneider Q, Ghezellou P, Seidel L, Bakonyi D, Will F, Spengler B, Zorn H, Gand M, Molecules, 2022, DOI:10.3390/molecules27196409.

[19]

Altering the chain-length specificity of a lipase from Pleurotus citrinopileatus for the application in cheese making.

Broel N, Sowa MA, Manhard J, Siegl A, Weichhard E, Zorn H, Li B, Gand M, Foods, 2022, DOI:10.3390/foods11172608

[18]

High throughput virtual screening for TCM against the main protease of SARS-CoV-2.

Qi X, Li B, Omarini AB, Gand M, Zhang X, Wang J, J Mol Struct, 2022, DOI: 10.1016/j.molstruc.2022.133709

[17]

Screening of fungi from the phylum Basidiomycota for degradation of boar taint aroma compounds

Omarini AB, Eloke JE, Fraatz MA, Mörlein D, Zorn H, Gand M, Eur Food Res Technol, 2022, DOI:10.1007/s00217-022-04045-4

[16]

Genome Mining-Guided Discovery and Characterization of the PKS-NRPS-Hybrid Polyoxyperuin produced by a Marine Streptomycete.

Kresna IDM, Wuisan Z, Pohl JM, Mettal U, Linares Otoya V, Gand M, Marner M, Linares Otoya L, Böhringer N, Vilcinskas A, Schäberle T, J Nat Prod, 2022, DOI:10.1021/acs.jnatprod.1c01018

[15]

Replacement of pregastric lipases in cheese production – Identification and heterologous expression of a lipase from Pleurotus citrinopileatus.

Sowa MA, Kreuter N, Sella N, Albuquerque W, Manhard J, Siegl A, Ghezellou P, Li B, Spengler B, Weichhard E, Rühl M, Zorn H, Gand M, J Agric Food Chem, 2022, DOI:10.1021/acs.jafc.1c07160.

[14]

Haze formation and the challenges for peptidases in wine protein fining.

Albuquerque W, Seidel L, Zorn H, Will F, Gand M, J Agric Food Chem, 2021, DOI: 10.1021/acs.jafc.1c05427

[13]

Genome and secretome analysis of Staphylotrichum longicolleum DSM105789 cultured on agro-residual and chitinous biomass.

Ali A, Ellinger B, Brandt SC, Betzel C, Rühl M, Wrenger C, Schlüter H, Schäfer W, Brognaro H, Gand M, Microorganisms, 2021, DOI:10.3390/microorganisms9081581

[12]

Identification of intact peptides by top-down peptidomics reveals cleavage spots in thermolabile wine proteins.

Albuquerque W, Ghezellou P, Li B, Spengler B, Will F, Zorn H, Gand M, Food Chem, 2021, DOI: 10.1016/j.foodchem.2021.130437

[11]

Insights into the genome and secretome of Fusarium metavorans DSM105788 by cultivation on agro-residual biomass and synthetic nutrient sources.

Brandt SC, Brognaro H, Ali A, Ellinger E, Maibach K, Rühl M, Wrenger C, Schlüter H, Schäfer W, Betzel C, Janssen S, Gand M, Biotechnol Biofuels 2021, 14:74, DOI: 10.1186/s13068-021-01927-9

[10]

Engineering a lipoxygenase from Cyclocybe aegerita towards long chain polyunsaturated fatty acids

Karrer D, Gand M, Rühl M, AMB Expr, 2021, 11:37, DOI: 10.1186/s13568-021-01195-8

[9]

Expanding the biocatalytic toolbox with a new type of ene/yne‐reductase from Cyclocybe aegerita

Karrer D, Gand M, Rühl M, ChemCatChem, 2021, 13, 1–10, DOI: 10.1002/cctc.202002011

[8]

Aspergillus sydowii: Genome Analysis and Characterization of Two Heterologous Expressed, Non-redundant Xylanases

Brandt SC, Ellinger B, van Nguyen T, Harder S, Schlüter H, Hahnke R, Rühl M, Schäfer W, Gand M, Front Microbiol, 2020, 11, 573482, DOI: 10.3389/fmicb.2020.573482

[7]

Promoter activation in Δhfq mutants as an efficient tool for specialized metabolite production enabling direct bioactivity testing

Bode E, Heinrich AK, Hirschmann M, Abebew D, Shi NY, Vo TD, Wesche F, Shi YM, Grün P, Simonyi S, Keller N, Engel Y, Wenski S, Bennet R, Beyer S, Bischoff I, Buaya A, Brandt SC, Cakmak I, Çimen H, Eckstein S, Frank D, Fürst R, Gand M, Geisslinger G, Hazir S, Henke M, Heermann R, Lecaudey V, Schäfer W, Schiffmann S, Schüffler A, Schwenk R, Skaljac M, Thines E, Thines M, Ulshöfer T, Vilcinskas A, Wichelhaus TA, Bode HB, Angew Chem Int Ed, 2019, 58(52), 18957-18963, DOI: 10.1002/anie.201910563

[6]

A unique fungal strain collection from Vietnam characterized for high performance degraders of bioecological important biopolymers and lipids

Brandt SC, Ellinger B, van Nguyen T, Thi QD, van Nguyen G, Baschien C, Yurkov A, Hahnke RL, Schäfer W, Gand M, PLoS One, 2018, 13(8), e0202695, DOI: 10.1371/journal.pone.0202695

[5]

One-step asymmetric synthesis of (R)- and (S)-rasagiline by reductive amination applying imine reductases

Matzel P, Gand M, Höhne M, Green Chem, 2017, 19(2), 385-389, DOI: 10.1039/C6GC03023H

[4]

A NADH- accepting imine reductase variant: Immobilization and cofactor regeneration by oxidative deamination

Gand M, Thöle C, Müller H, Brundiek H, Bashiri G, Höhne M, J Biotechnol, 2016, 230, 11-18, DOI: 10.1016/j.jbiotec.2016.05.006

[3]

Asymmetric reductive amination of ketones catalyzed by imine reductases

Wetzl D, Gand M, Ross A, Müller H, Matzel P, Hanlon SP, Müller M, Wirz B, Höhne M, Iding H, ChemCatChem, 2016, 8(12), 2023-2026, DOI: 10.1002/cctc.201600384

[2]

Enhancement of promiscuous amidase activity of a Bacillus subtilis esterase by formation of a Pi-Pi network

Hackenschmidt S, Moldenhauer E, Behrens G, Gand M, Pavlidis I, Bornscheuer U, ChemCatChem, 2014, 6(4), 1015-1020, DOI: 10.1002/cctc.201300837

[1]

Characterization of three novel enzymes with imine reductase activity

Gand M, Müller H, Wardenga R, Höhne M, J Mol Catal B Enzym, 2014, 110, 126-132, DOI: 10.1016/j.molcatb.2014.09.017

 

Publikationen: Books and book chapters

[1]

Asymmetric Reduction of C=N Bonds by Imine Reductases and Reductive Aminases

Höhne M, Matzel P, Gand M, in Pharmaceutical Biocatalysis: Chemoenzymatic Synthesis of Active Pharmaceutical Ingredients, (Ed: P. Grunwald), 2019, Jenny Stanford Publishing, Singapore.

 

Publikationen: Non-peer-reviewed journals

[3]

Biotechnologie als Chance für eine nachhaltige Lebensmittelproduktion

Fischer L, Berger RG, Bisping B, Eisele T, Ersoy F, Gand M, Geißler T, Hellmuth K, Hertel C, Hitzmann, B, Jany KD, Kiy T, Lorenz P, Manhard J, Marx S, Rabenhorst J, Stressler T, von Wallbrunn C, Zhang Y, Zorn H, 2022, DECHEMA, https://dechema.de/dechema_media/Downloads/Positionspapiere/Biotechnologie+f%C3%BCr+nachhaltige+Lebensmittelproduktion.pdf

[2]

Lipases from edible mushrooms for the production of cheese.Sowa MA, Gand M, Manhard J, Siegl A, 2022, International Dairy Magazine, https://www.international-dairy.com/blmedien-epaper/idm-international-dairy-magazine-03-04-2022/

[1]

Asymmetric Reduction of C=N Bonds by Imine Reductases and Reductive Aminases

M. Höhne, P. Matzel, M. Gand, in Pharmaceutical Biocatalysis: Chemoenzymatic Synthesis of Active Pharmaceutical Ingredients, (Ed: P. Grunwald), 2019, Jenny Stanford Publishing, Singapore.