Virological and serological tests for small and wild ruminants
(SM: sample material; FD: frequency and duration of test; ‡ not accredited)
Border disease virus (BDV) and Pestiviruses in wild ruminants (deer)
Virus detection
- Virus isolation (TC)
SM: blood with anticoagulant, swabs, organs
FD: daily/ 7-9 days
- Genome detection (RT-PCR)
SM: blood with anticoagulant, swabs, organs
FD: daily/ 48h
Antibody detection
- Serum neutralisation test (SNT; Border Disease)
SM: serum, 1 ml
FD: on demand/ 5-7 days
Small ruminant lentiviruses / Maedi-Visna & Caprine Arthritis and Encephalitis
Virus detection
- Genome detection (RT-PCR)‡
SM: organs (lung, CNS)
FD: daily/ ca. 48h
Antibody detection
- competitive ELISA
SM: serum, 1 ml
FD: daily/ ca. 24h
Coronavirus and Rotavirus/ Diarrhea
Virus detection
- Virus particle detection (EM)
SM: faecal samples, organs
FD: daily/ ca. 48h
- Genome detection (RT-PCR)‡
SM: faecal samples, organs
FD: daily/ ca. 48h
Malignant catarrhal fever, persistent infections / Ovine/Caprine herpesvirus 2 (OvHV-2/CpHV-2), Alcelaphine Herpesvirus 1 (AlHV-1)
Virus detection
- Genome detection (PCR)
SM: Blood with anticoagulant, nasal/ conjunctival swabs, organs
FD: daily/ ca. 48h
- Quantitative genome detection (Real-Time PCR)
SM: Blood with anticoagulant, nasal/ conjunctival swabs, organs
FD: on demand/ ca. 48h
Antibody detection
- Serum neutralisation test (AlHV, cross reaction)
cannot be offered at the moment
Parapoxvirus/ Ecthyma contagiosum, ORF
Virus detection
- Virus particle detection (EM)
SM: skin scrapes
FD: daily/ ca. 48h
- Genome detection (PCR)‡
SM: skin scrapes
FD: daily/ ca. 48h
Ovine pulmonary adenocarcinoma / Jaagsiekte sheep retrovirus (JSRV)
Virus detection
- Genome detection (RT-PCR) ‡
SM: lung, nasal swab
FD: daily/ ca. 48h
Bovine Norovirus / Diarrhea
Virus detection
- Genome detection (RT-PCR)
SM: faeces
FD: daily/ ca. 48h
- Virus particle detection (EM)
SM: faeces
FD: daily/ ca. 48h
Bluetongue Virus Serotyp 8 (BTV)
Virus detection
- Genome detection (RT-PCR)
SM: blood with anticoagulant, organs
FD: daily/ ca. 48h
Schmallenbergvirus (SBV)
Virus detection
- Genome detection (RT-PCR)‡
SM: blood with anticoagulant, organs
FD: daily/ ca. 48h
Borna Disease / BoDV
Virus detection
- Genome detection (RT-PCR)
SM: organs (CNS)
FD: daily/ ca. 48h
Antibody detection
- Immunofluorescence test
SM: serum, CSF (0.5ml)
FD: Tue, Thu/ 24h
Papillomavirus / Papillomatosis
Virusnachweis
- Virus particle detection (EM)
SM: warts
FD: daily/ ca. 48h
- Genome detection (PCR)‡
SM: warts
FD: daily/ ca. 48h
- Vaccine production‡
SM: warts (at least. 1g / 0.4g per animal)
FD: daily/ ca. 10-14 days