Tanja Rehling

Office: TIG / 2.40

Tel.: +49 641/99-35903

tanja.rehling

Justus Liebig University Giessen

Institute for Insect Biotechnology

Department of Insect Biotechnology in Plant Protection

Winchester Str. 2

35394 Giessen

Curriculum Vitae

Research Interests

Current project:

Since April 2014, in the group of Prof. Dr. Marc F. Schetelig. The group develops and evaluates transgenic insects, such as mosquitos and fruit flies, for use in insect pest control programs. My work and responsibilities include Mosquito rearing, Mosquito germline transformation via embryonic microinjection, genetic crosses and screening for transgenics, molecular sequence analysis, inverse PCR, expression analysis with PCR, integration analysis, digital droplet PCR, creating cloning strategies, cloning of plasmid vectors, design of guideRNA for CRISPR-Cas9 experiments, cell culture of insect cell lines and experiments, biochemical techniques and immunohistochemistry in insects, passing practices to students and newcomers.

 

Professional experience:                       

Since October 2019 part-time study: Bachelor study "Molecular Biology", Johannes Gutenberg-University Mainz.

From February 2011 to April 2014, as a technical assistant in the project SFB/Transregio 79 – „Materials for Tissue Regeneration in Systemically Diseased Bones“ in the Laboratory of Experimental Trauma Surgery, Justus-Liebig-University, Giessen. The work focused on developing suitable animal models and testing newly developed biomaterials. This work included animal surgeries and euthanasia, collecting tissue and bone samples, sectioning,  histological, immunohistochemical, and enzyme staining, and their histomorphometric evaluation. Additionally, the development and validation of PCR and qPCR studies for gene expression analysis of markers of bone formation and resorption and evaluation of the results for the publications was a large part of the work.

From August 1987 to February 2011 as technical assistant in clinical and physiological research and studies at the Max-Planck-Gesellschaft, Clin. Res. Unit for Blood Coagulation, Giessen and Max-Planck-Institute for Clinical and Physiological Research, Bad Nauheim. This work involved cloning of plasmids, transformation, transfection, expression in cell lines, and evaluation of the recombinant coagulation factors as well as purification and characterization of proteins from plasma, preparation of monoclonal and polyclonal antibodies, screening of patients for genetic defects associated with thrombophilia and clinical diagnosis in the central laboratory of the Kerckhoff Clinic.

 

Publications

Häcker I, Rehling T, Schlosser H, Mayorga-Ch D, Heilig M, Yan Y, Armbruster PA, Schetelig MF (2023). Improved piggyBac transformation with capped transposase mRNA in Pest Insects. International Journal of Molecular Sciences; 24(20):15155.

Yan Y, Hosseini B, Scheld A, Pasham S, Rehling T, Schetelig MF (2023). Effects of antiviotics on the in vitro expression of tetracycline-off constructs and the performance of Drosophila suzukii female-killing strains. Frontiers in Bioengineering and Biotechnology, 14:11:876492.

Häcker I, Koller R, Eichner G, Martin J, Liapi E, Rühl J, Rehling T, Schetelig MF (2020). Evaluation of Hydrogen Peroxide Fumigation and Heat Treatment for Standard Emergency Arthropod Inactivation in BSL-3 Insectaries. Frontiers in Bioengineering and Biotechnology, 16;8:602937.

Ramírez-Santos E, Rendón P, Bourtzis K, Schetelig MF, Cáceres C, Targovska A, Rehling T, Guillén K, Ruiz-Montoya L, Toledo J, Liedo P (2018). Generational interaction of a transgenic Ceratitis capitata (Tephritidae) strain and the parasitoid, Fopius ceratitivorus (Braconidae), as a model to assess the risk of horizontal gene transfer in eukaryotes. PLoS One 13(12): e0207999 

Thormann U, Ray S, Sommer U, El Khassawna T, Rehling T, Hundgeburth M, Henß A, Rohnke M, Janek J, Lips KS, Heiss C, Schlewitz G, Szalay G, Schumacher M, Gelinsky M, Schnettler R, Alt V (2013). Bone formation induced by strontium modified calcium phosphate cement in critical-size metaphyseal fracture defects in ovariectomized rats. Biomaterials 34(34): 8589–8598.